返回顶部基本信息
| 姓名 | 黄耀伟 |  |
| 职称 | 教授 | |
| 职务 | ||
| 导师类别 | 博士生导师 | |
| 招生方向 | 分子病毒学、病毒新型疫苗研发 | |
| 联系方法 | E-mail: yhuang@zju.edu.cn | |
| 地址：浙江大学紫金港校区动物科学学院E543 | ||
| 个人主页 | http://mypage.zju.edu.cn/yaoweihuang |
主要学术成果：
迄今发表SCI收录论文46篇，包括发表在mBio，Journal of Virology，Virology等病毒学领域一流期刊；总引用470余次（根据ISI数据库），H因子13；其中第一作者论文14篇（含通讯作者1篇），包括应邀在Virus Research上撰写猪繁殖与呼吸综合征病毒（PRRSV）疫苗研发展望的综述文章。申报美国及欧亚拉美多国猪病毒疫苗相关专利3项，已授权1项；中国授权鸡病毒疫苗专利两项。获浙江省人民政府科技进步一等奖2次（分别排名第2和第3）。Virology & Mycology编委（Editorial Board Member） 。
返回顶部教育背景与工作简历
教育经历：
2000/09—2003/03，浙江大学，生物医学工程系，博士学位；
1998/09—2000/08，浙江大学，动物科学学院，攻读预防兽医学硕士（后转硕博连读）；
1994/09—1998/07，南京大学，生物科学与技术系，学士学位
研究工作经历：
2013/09— 浙江大学动物科学学院，教授
2011/01—2013/08，美国弗吉尼亚理工大学兽医学院生物医学与疾病生物学系，研究助理教授(Research Assistant Professor)
2008/01—2010/12，美国弗吉尼亚理工大学兽医学院，研究科学家(Research Scientist)
2003/04—2007/12，美国弗吉尼亚理工大学兽医学院，博士后
返回顶部学术兼职
返回顶部所获荣誉与主要奖励
返回顶部研究领域
从事人兽共患病毒、新生病毒和猪重要致病性病毒的基础与应用研究，主要包括人、猪和鸡戊型肝炎病毒（HEV），猪繁殖与呼吸综合征病毒（PRRSV），猪流行性腹泻病毒（PEDV），人、猪细环病毒（TTSuV），猪圆环病毒2型（PCV2），猪树突状细胞生物学以及猪病毒新型疫苗的研发。目前研究重点为PEDV和人HEV。
返回顶部完成及现有课题
Research grants (as Co-PI) in USA:
1. Title: A chicken model to study HEV pathogenesis
Funding Agency: National Institutes of Health (NIH) R01
Duration: 03/2008 to 05/2012
2. Title: Mechanism of hepatitis E virus replication and pathogenesis
Funding Agency: NIH R01
Duration: 04/2008 to 05/2012
3. Title: A Molecular Approach to Propagate Swine Torgue teno virus (TTV) in Cell Culture and Definitively Characterize the Virus in Pigs.
Funding Agency: Boehringer-Ingelheim Vetmedica Inc.
Duration: 01/2009 to 08/2012
4. Title: Engineering PRRSV Vaccines that confer heterologous protection.
Funding Agency: USDA
Duration: 12/15/2010 to 12/14/2014
5. Title: Development of a new generation improved modified-live attenuated PRRSV vaccine.
Funding Agency: Pfizer Inc.
Duration: 10/01/2010-09/31/2013
6. Title: Systematic identification and analyses of microRNA and messenger RNA profiles in HepG2 cells infected with a cell culture-adapted hepatitis E virus (HEV).
Funding Agency: Virginia Bioinformatics Institute.
Duration: 09/01/2010-05/31/2013
7. Title: Mechanism of hepatitis E virus replication and pathogenesis.
Funding Agency: NIH R01 (renewed)
Duration: 07/2013 to 06/2017
返回顶部学术论文
PUBLICATIONS (identified by ISI Science Citation Index/Web of Science)
红色字体文章为具有相对重要影响的研究工作。
First author publications listed first (nos.1-14):
1. Huang YW*, Dickerman AW, Piñeyro P, Li L, Fang L, Kiehne R, Opriessnig T, Meng XJ*. Origin, Evolution, and Genotyping of Emergent Porcine Epidemic Diarrhea Virus Strains in the United States. mBio, 2013, 4(5):e00737-13. (*Corresponding author)
2. Huang YW, Harrall KK, Dryman BA, Opriessnig T, Vaughn EM, Roof MB, Meng XJ. Serological profile of Torque teno sus virus species 1 (TTSuV1) in pigs and antigenic relationships between two TTSuV1 genotypes (1a and 1b), between two species (TTSuV1 and 2), and between porcine and human anelloviruses. Journal of Virology, 2012, 86(19):10628-10639.
3. Huang YW, Patterson AR, Opriessnig T, Dryman BA, Gallei A, Harrall KK, Vaughn EM, Roof MB, Meng XJ. Rescue of a porcine anellovirus (Torque teno sus virus 2) from cloned genomic DNA in pigs. Journal of Virology, 2012, 86(11):6042-6054.
4. Huang YW, Harrall KK, Dryman BA, Beach NM, Kenney SP, Opriessnig T, Vaughn EM, Roof MB, Meng XJ. Expression of the putative ORF1 capsid protein of Torque teno sus virus 2 (TTSuV2) and development of Western blot and ELISA serodiagnostic assays: Correlation between TTSuV2 viral load and IgG antibody level in pigs. Virus Research, 2011, 158(1-2):79-88.
5. Huang YW, Ni YY, Dryman BA, Meng XJ. Multiple infection of porcine Torque teno virus in a single pig and characterization of the full-length genomic sequences of four U.S. prototype PTTV strains: implication for genotyping of PTTV. Virology, 2010, 396(2):289-297.
6. Huang YW, Meng XJ. Novel strategies and approaches to develop the next generation of vaccines against porcine reproductive and respiratory syndrome virus (PRRSV) (Invited Review). Virus Research, 2010, 153(1-2):141-149
7. Huang YW, Dryman BA, Harrall KK, Vaughn EM, Roof MB, Meng XJ. Development of SYBR green-based real-time PCR and duplex nested PCR assays for quantitation and differential detection of species- or type-specific porcine Torque teno viruses. Journal of Virological Methods, 2010, 170(1-2):140-146
8. Huang YW, Dryman B, Li W, Meng XJ. Porcine DC-SIGN: Molecular cloning, gene structure, tissue distribution and binding characteristics. Developmental and Comparative Immunology, 2009, 33(4):464-480.
9. Huang YW, Fang Y, Meng XJ. Identification and characterization of a porcine monocytic cell line supporting porcine reproductive and respiratory syndrome virus (PRRSV) replication and progeny virion production by using an improved DNA-launched PRRSV reverse genetics system. Virus Research, 2009, 145(1):1-8.
10. Huang YW, Meng XJ. Identification of a porcine DC-SIGN-related C-type lectin, porcine CLEC4G (LSECtin) and its order of intron removal during splicing: Comparative genomic analyses of the cluster of genes CD23/CLEC4G/DC-SIGN among mammalian species.Developmental and Comparative Immunology, 2009, 33(6):747-760.
11. Huang YW, Opriessnig T, Halbur PG, Meng XJ. Initiation at the third in-frame AUG codon of open reading frame 3 of the hepatitis E virus is essential for viral infectivity in vivo.Journal of Virology, 2007, 81(6):3018-3026.
12. Huang YW, Haqshenas G, Kasorndorkbua C, Halbur PG, Emerson SU, Meng XJ. Capped RNA transcripts of full-length cDNA clones of swine hepatitis E virus are replication competent when transfected into Huh7 cells and infectious when intrahepatically inoculated into pigs. Journal of Virology, 2005,79(3):1552-1558.
13. Huang YW, Li L, Li JR, Yu L. Rapid construction of infectious clones of infectious bursal disease virus. Acta Biochimica et Biophysica Sinica, 2003, 35(4):338-344.
14. Huang YW, Yu L, Ding HM, Li JR, Song KH. Amplification and cloning by long RT-PCR of full-length genome of larger segment of chicken infectious bursal disease viruse. Acta Biochimica et Biophysica Sinica, 2001, 33(3):355-359.
15. Zhu Y, Yu X, Zhang Y, Ni Y, Si F, Yu R, Dong S, Huang Y, Li Z. Infectivity of a genotype 4 hepatitis E virus cDNA clone by intrahepatic inoculation of laboratory rats. Veterinary Microbiology, 2013,166(3-4):405-411.
16. Zhou L, Ni YY, Piñeyro P, Cossaboom CM, Subramaniam S, Sanford BJ, Dryman BA,Huang YW, Meng XJ. Broadening the heterologous cross-neutralizing antibody inducing ability of porcine reproductive and respiratory syndrome virus by breeding the GP4 or M genes. PLoS One, 2013, 8(6):e66645.
17. Ni YY, Opriessnig T, Zhou L, Cao D, Huang YW, Halbur PG, Meng XJ. Attenuation of porcine reproductive and respiratory syndrome virus by molecular breeding of the virus envelope genes from genetically divergent strains. Journal of Virology, 2013, 87(1):304-313.
18. Zhou L, Ni YY, Piñeyro P, Sanford BJ, Cossaboom CM, Dryman BA, Huang YW, Cao DJ, Meng XJ. DNA shuffling of the GP3 genes of porcine reproductive and respiratory syndrome virus (PRRSV) produces a chimeric virus with an improved cross-neutralizing ability against a heterologous PRRSV strain. Virology, 2012, 434(1):96-109.
19. Cordoba L, Feagins AR, Opriessnig T, Cossaboom CM, Dryman BA, Huang YW, Meng XJ. Rescue of a genotype 4 human hepatitis E virus (HEV) from cloned cDNA and characterization of intergenotypic chimeric viruses in cultured human liver cells and in pigs.Journal of General Virology, 2012, 93(10):2183-2194.
20. Kenney SP, Pudupakam RS, Huang YW, Pierson FW, Leroith T, Meng XJ. The PSAP motif within the ORF3 protein of an avian strain of the hepatitis E virus is not critical for viral infectivity in vivo but plays a role in virus release. Journal of Virology, 2012, 86(10):5637-5646.
21. Xiao CT, Giménez-Lirola L, Huang YW, Meng XJ, Halbur PG, Opriessnig T. The prevalence of Torque teno sus virus (TTSuV) is common and increases with the age of growing pigs in the United States. Journal of Virological Methods, 2012, 183(1):40-44.
22. Cordoba L, Huang YW, Opriessnig T, Harral KK, Beach NM, Finkielstein C, Emerson SU, Meng XJ. Three amino acid mutations (F51L, T59A and S390L) on the capsid protein of the hepatitis E virus collectively contribute to virus attenuation. Journal of Virology, 2011, 85(11):5338-5349.
23. Pudupakam RS, Kenney SP, Córdoba L, Huang YW, Dryman BA, Leroith T, Pierson FW, Meng XJ. Mutational Analysis of the Hypervariable Region of the Hepatitis E Virus Reveals Its Involvement in the Efficiency of Viral RNA Replication. Journal of Virology, 2011, 85(19):10031-10040.
24. Ni YY, Huang YW, Cao D, Opriessnig T, Meng XJ. Establishment of a DNA-launched infectious clone for a highly pneumovirulent strain of type 2 porcine reproductive and respiratory syndrome virus: Identification and in vitro and in vivo characterization of a large spontaneous deletion in the nsp2 region. Virus Research, 2011, 160(1-2):264-273.
25. Opriessnig T, Shen HG, Pal N, Ramamoorthy S, Huang YW, Lager KM, Beach NM, Halbur PG, Meng XJ. A live-attenuated chimeric porcine circovirus type 2 (PCV2) vaccine is transmitted to contact pigs but is not upregulated by concurrent infection with porcine parvovirus (PPV) and porcine reproductive and respiratory syndrome virus (PRRSV) and is efficacious in a PCV2b-PRRSV-PPV challenge model. Clinical and Vaccine Immunology, 2011, 18(8):1261-1268.
26. Opriessnig T, Gomes-Neto JC, Hemann M, Shen HG, Beach NM, Huang YW, Halbur PG, Meng XJ. An experimental live chimeric porcine circovirus 1-2a vaccine decreases porcine circovirus 2b viremia when administered intramuscularly or orally in a porcine circovirus 2b and porcine reproductive and respiratory syndrome virus dual-challenge model. Microbiology and Immunology, 2011, 55(12):863-873.
27. Sinha A, Shen HG, Schalk S, Beach NM, Huang YW, Meng XJ, Halbur PG, Opriessnig T. Porcine reproductive and respiratory syndrome virus (PRRSV) influences infection dynamics of porcine circovirus type 2 (PCV2) subtypes PCV2a and PCV2b by prolonging PCV2 viremia and shedding. Veterinary Microbiology, 2011, 152(3-4):235-246.
28. Sanford BJ, Dryman BA, Huang YW, Feagins AR, Leroith T, Meng XJ.Prior infection of pigs with a genotype 3 swine hepatitis E virus (HEV) protects against subsequent challenges with homologous and heterologous genotypes 3 and 4 human HEV. Virus Research, 2011,159(1):17-22.
29. Cao D, Huang YW, Meng XJ. The nucleotides on the stem-loop RNA structure in the junction region of the hepatitis E virus (HEV) genome are critical for virus replication. Journal of Virology, 2010, 84(24):13040-13044.
30. Kwon HM, LeRoith T, Pudupakam RS, Pierson FW, Huang YW, Dryman BA, Meng XJ. Construction of an infectious cDNA clone of avian hepatitis E virus (avian HEV) recovered from a clinically healthy chicken in the United States and characterization of its pathogenicity in specific-pathogen-free chickens. Veterinary Microbiology, 2011, 147(3-4):310-319.
31. Feagins AR, Cordoba L, Sanford BJ, Dryman BA, Huang YW, LeRoith T, Emerson SU, Meng XJ. Intergenotypic chimeric hepatitis E viruses (HEV) with the genotype 4 human HEV capsid protein and genotype 3 swine HEV backbone are infectious in pigs. Virus Research, 2011, 156(1-2):141-146.
32. Sinha A, Shen HG, Schalk S, Beach NM, Huang YW, Halbur PG, Meng XJ, Opriessnig T. Porcine reproductive and respiratory syndrome virus (PRRSV) infection at the time of porcine circovirus type 2 (PCV2) vaccination has no impact on vaccine efficacy. Clinical and Vaccine Immunology. 2010, 17(12):1940-1945.
33. Shen HG, Beach N, Huang YW, Halbur PG, Meng XJ, Opriessnig T. Comparison of commercial and experimental porcine circovirus type 2 (PCV2) vaccines using a triple challenge with PCV2, porcine reproductive and respiratory syndrome virus (PRRSV), and porcine parvovirus (PPV). Vaccine, 2010, 28(37):5960-5966.
34. Ramamoorthy S, Huang FF, Huang YW, Meng XJ. Interferon-mediated enhancement of in vitro replication of porcine circovirus type 2 is influenced by an interferon stimulated response element in the PCV2 genome. Virus Research, 2009, 145(2):236-243.
35. RS Pudupakam, YW Huang, T Opriessnig, PG Halbur, FW Pierson, XJ Meng. Deletions of the hypervariable region (HVR) in the open reading frame 1 of hepatitis E virus (HEV) do not abolish virus infectivity: Evidence for attenuation of the HVR-deletion mutants in vivo.Journal of Virology, 2009, 83(1):384-395.
36. AR Feagins, T Opriessnig, YW Huang, PG Halbur, XJ Meng. Cross-species infection of specific-pathogen-free pigs 1 by a genotype 4 strain of human hepatitis E virus. Journal of Medical Virology, 2008, 80(8):1379–1386.
37. N Pal, YW Huang, DM Madson, C Kuster, XJ Meng, PG Halbur, T Opriessnig. Development and validation of a duplex real-time PCR assay for the simultaneous detection and quantification of porcine circovirus type 2 and an internal control on porcine semen samples. Journal of Virological Methods, 2008, 149(2):217-225.
38. Matanin BM, Huang Y, Meng XJ, Zhang C. Purification of the major envelop protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions. Journal of Virological Methods, 2008, 147(1):127-135.
39. Li L, Fang W, Li J, Huang Y, Yu L. Oral DNA vaccination with the polyprotein gene of infectious bursal disease virus (IBDV) delivered by the attenuated Salmonella elicits protective immune responses in chickens. Vaccine. 2006, 24(33-34):5919-5927.
40. Li L, Wei Y, Huang Y, Yu L. Generation of VP5 deficient mutant of infectious bursal disease virus strain HZ2. Chinese Science Bulletin. 2006, 51(15):1909-1912.
41. Li L, Huang YW, Wang LS, Wan WJ, Yu L. Synthesis of reassortant infectious bursal disease virus in chickens injected directly with infectious clones from different virus strains.Acta Biochimica et Biophysica Sinica, 2005, 37(3):192-198.
42. Li J, Liang X, Huang Y, Meng S, Xie R, Deng R, Yu L. Enhancement of the immunogenicity of DNA vaccine against infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin 2. Virology, 2004, 329(1):89-100.
43. Li J, Huang Y, Liang X, Lu M, Li L, Yu L, Deng R. Plasmid DNA encoding antigens of infectious bursal disease viruses induce protective immune responses in chickens: factors influencing efficacy. Virus Research, 2003, 98(1):63-74.
44. Yu L, Li JR, Huang YW, Dikki J, Deng R. Molecular characteristics of full-length of genomic segment A of three infectious bursal disease viruses in China: two attenuated strains and a field virulent strain. Avian Diseases, 2001, 45(4):862-874.
45. Li JR, Huang YW, Meng SS, Liang XY, Xie RH, Yu L. Cloning and phylogenetic analysis of interleukin-2 gene in Xiaoshan chicken, a Chinese local chicken breed. Acta Biochimica et Biophysica Sinica, 2001, 33(6):713-718.
46. Song KH, Jin YF, Huang YW, Zhang YZ, Yu L. Infectious bursal disease virus structural protein VP2 expressed by a baculovirus recombinant in Bombyx mori. Acta Biochimica et Biophysica Sinica, 2000, 32(3):281-284.
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欢迎报考2014年硕士、博士研究生 and 欢迎病毒学、分子生物学、预防兽医学方向博士毕业生应聘博士后：
对本实验室研究感兴趣的同学请与我联系：电子邮件 yhuang at zju.edu.cn或yhuang at vt.edu；可预约面谈。办公室地址：浙江大学紫金港校区动物科学学院E543室。 本课题组与美国弗吉尼亚理工大学兽医学院、俄亥俄州立大学兽医学院、爱荷华州立大学兽医学院、英国爱丁堡大学罗斯林研究所有联合合作课题并紧密联系，作出突出成果的同学和老师将大力推荐到上述院校继续深造和交流合作。