成果展示

Depot-specific differences in fat mass expansion in WT and ob/ob mice

编辑： 时间：2018年09月08日 访问次数:234

Depot-specific differences in fat mass expansion in WT and ob/ob mice

Xinxia Wang（王新霞）,#1 Caihua Yu,#1 Jie Feng（冯杰）,1 Jin Chen,1 Qin Jiang,1 Shihuan Kuang,2 and Yizhen Wang（汪以真）1
1 College of Animal Sciences, Zhejiang University, Key Laboratory of Animal Nutrition & Feed Sciences, Ministry of Agriculture, Zhejiang Provincial Laboratory of Feed and Animal Nutrition, Hangzhou, Zhejiang 310058, P. R. China
2 Department of Animal Sciences and Center for Cancer Research, Purdue University, West Lafayette, IN 47907, USA
#Contributed equally.


期刊：ONCOTARGET

Impact Factor (影响因子): 5.16（2016年）

出版年：JUL 2017

摘要：The study was designed to investigate the cellular mechanisms underlying the differential fat expansion in different fat depots in wild type (WT) and ob/ob (OB) mice. At 6 weeks old, no differences in fat mass were found between SAT and VAT in WT mice, while O-SAT showed significantly higher weight than that of O-VAT. The average adipocyte size of SAT (～ 4133.47 μm2) was smaller than that of VAT (～ 7438.91 μm2) in OB mice. O-SAT preadipocytes gained higher triglyceride contents and higher levels of PPARγ and C/EBPα than did O-VAT preadipocytes upon in vitro differentiation. W-SAT and W-VAT displayed no significant differences in fatty acid uptake, while 1.36 fold significantly higher fatty acid uptake was found in O-SAT compared to O-VAT. Approximately 52% of the radioactivity recovered in cellular lipids was found in TAG in O-SAT, which was significantly higher than the other three adipocyte types. Significantly more radiolabelled oleic acid was β-oxidized to CO2 in adipocytes from O-VAT than that from O-SAT. ATP production was significantly lower in W-SAT compared with W-VAT, whereas no significantly ATP level was observed between O-SAT and O-VAT. Expression of UCP-1 in SAT from either WT or OB mice was significantly higher than the counterpart of VAT, which demonstrated higher uncoupled respiration and lower oxidative phosphorylation in SAT. Together, a combined increase in adipogenesis and FA uptake, and decreases in β-oxidation and ATP production, contributed to greater expansion of SAT compared to VAT in obese mice.