Department of Veterinary Medicine
Associate Professor of Virology and Immunology
Tel: +86-571-86971092
Fax: +86-571-86971821
E-mail: guojq@zju.edu.cn
Address:
258# Animal Science Building
Zhejiang University
268 Kaixuan Road
Hangzhou, Zhejiang 310029
P. R. China
Research Interests:
1. Molecular Virology and Immunology
2. Antibody Engineering
Educations:
2000-2003 PhD (Microbiology), College of Life Sciences, Wuhan University, Wuhan, P. R. China.
1993-1996 Master of Agriculture (Preventive Veterinary Medicine), College of Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou, P. R. China.
1989-1993 Bachelor of Agriculture (Veterinary Medicine), College of Husbandry and Veterinary Medicine, Henan Agricultural University, Zhengzhou, P. R. China.
Brief Chronology of Employment
2006- Associate professor of immunology, Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, P. R. China.
2003-2005 Post-doctoral researcher, Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, P. R. China.
1996-2000 Research Assistant, Institute of Biotechnology, Hanan Academy of Agricultural Sciences, Zhengzhou, P. R. China.
Publications:
1. Zhang GP, Guo JQ (equal contribution), Zhou JY, Wang XN, Li QM, Yang YY, Shen HG, Zhao D, Zhang H, Xi J, Wang L, Qiao SL and Jin X. 2006. Identification of the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFc??2R) using synthetic peptides. FEBS Lett. 580(2): 1383-1390.
2. Guo JQ, Li QM, Zhou JY, Zhang GP, Yang YY, Xing GX, Zhao D, You SY and Zhang CY. 2006. Efficient recovery of the functional IP10-scFv fusion protein from inclusion bodies with an on-column refolding system. Protein Expr. Purif. 45(1): 168-174.
3. Chen HX, Shen HG, Li XL, Zhou JY, Hou YQ, Guo JQ and Hu JQ. 2006. Seroprevalance and identification of influenza a virus infection from migratory wild waterfowl in china (2004-2005). J. Vet. Med. B Infect. Dis. Vet. Public Health 53(4): 166-170.
4. Zhang GP, Guo JQ, Wang XN, Yang JX, Yang YY, Li QM, Li XW, Deng RG, Xiao ZJ, Yang JF, Xing GX and Zhao D. 2006. Development and evaluation of an immunochromatographic strip for trichinellosis detection. Vet. Parasitol. 137(3-4): 286-293.
5. Zhang GP, Wang XN, Yang JF, Yang YY, Xing GX, Li QM, Zhao D, Chai SJ and Guo JQ. 2006. Development of an immunochromatographic lateral flow test strip for detection of ??-adrenergic agonist clenbuterol residues. J. Immunol. Methods (In Press)
6. Zhang GP, Li QM, Yang YY, Guo JQ, Li XW, Deng RG, Xiao ZJ, Xing GX, Yang JF, Zhao D, Cai SJ and Zang WM. 2005. Development of a one-step strip test for the diagnosis of chicken infectious bursal disease. Avian Dis. 49: 177-181.
7. Zhou JY, Ye JX, Ye WC, Chen QX, Zheng XJ and Guo JQ. 2005. Antigenic and molecular characterization of infectious bursal disease virus in china from layer chicken flocks. Prog. Biochem. Biophys. 32: 37-45.
8. Zhou JY, Wang JY, Chen JG, Wu JX, Gong H, Teng QY, Guo JQ, Shen HG. 2005. Cloning, in vitro expression and bioactivity of duck interleukin-2. Mol. Immunol. 42: 589-598.
9. Wang XN, Zhang GP, Zhou JY, Feng CH, Yang YY, Li QM, Guo JQ, Qiao HX, Xi J, Zhao D, Xing GX, Wang L, Wang SH, Xiao ZJ, Li XW and Deng RG. 2005. Identification of neutralizing epitopes on the VP2 protein of infectious bursal disease virus by phage displayed heptapeptide library screening and synthetic peptide mapping. Viral Immunol. 18(3): 549-557.
10. Zhou JY, Shang SB, Gong H, Chen QX, Wu JX, Shen HG, Chen TF and Guo JQ. 2005. In vitro expression, monoclonal antibody and bioactivity for capsid protein of porcine circovirus type II without nuclear localization signal. J. Biotechnol. 118(2): 201-211.
11. Guo JQ, Chen L, Ai HW, Jing JN, Zhou JY, Zhang CY and You SY. 2004. A novel fusion protein of IP10-scFv retains antibody specificity and chemokine function. Biochem. Biophys. Res. Commun. 320(2): 506-513.
12. Guo JQ, You SY, Li L, Huang JN, Zhang YZ and Zhang CY. 2003. Construction and high-level expression of a single-chain Fv antibody fragment specific for acidic isoferritin in Escherichia coli. J. Biotech. 102(2): 177-189.
13. Xiao M, Zhang CY, Pan ZS, Wu HX, Guo JQ. 2002. Classical swine fever virus NS5B-GFP fusion protein possesses an RNA-dependent RNA polymerase activity. Arch. Virol. 147(9): 1779-1787.
14. Sheng HG, Zhou JY, Chen QX, Guo JQ, Chen TF, Shang SB and Li ZK. 2005. Expression and characterization of porcine circovirus type II rep gene in PK-15 cells. Chin. J. Vet. Sci. 25(3): 244-246??
15. You SY, Guo JQ, Ai HW and Chen L. 2005. Preparation and characterization of anti-acidic isoferritin recombinant immunocytokine. Chin. J. Microbiol. Immunol. 25(8): 625-629.
16. Zhang GP, Xi J, Wang XN, Qiao HX, Zhang H, Wang L and Guo JQ. 2005. Expression and Identification of IBDV VP3 Structural Protein in E. coli. J. Henan Agri. Sci. 8: 88-91.
17. Zhang GP, Wang L, Li QM, Zhang H, Qiao SL, Qiao HX, Xi J, Wang XN, Guo JQ. Expression and identification of the extracellular domain of bovine Fc??RII in E. coli. J. Henan Agri. Sci. 7: 86-89.
18. Zhang GP, Qiao SL, Li QM, Wang L, Zhang H, Guo JQ, Wang XN and Xia C. 2005. Expression of bovine Fc??RI (CD64)-GST fusion protein in E. coli. J. Henan Agri. Sci. 6: 79-82.
19. Qiao SL, Zhang GP, Wang L, Zhang H, Zhang H, Fu YF, Guo JQ, Wang XN and Xia C. 2005. Cloning of bovine Fc??2R cDNA with high GC content by PCR. J. Henan Agri. Sci. 5: 67-69.
20. Wu HX, Zhang CY, Zheng CY and Guo JQ. 2001. Genomic sequence determination of classical swine fever virus persistent infection strain. Wuhan University J. Nat. Sci. 26: 864-866.
21.Wang CQ, Liang HD, Guo JQ, Qiao HX, Wang YB, Zuo XT, Xiong SH, Lu JF, Liu HY and Jin Y. 2001. Comparison of IBDV antigen titers between bursa and embryo detected by different methods. Chin. J. Preventive Vet. Med. 23: 458-460.
22. Guo JQ, Zhang GP, Yang YY, Li QM, Wang AP, Yan YH, Li XW and Deng RG. 2000. Development and application of the diagnostic kit of infectious bursal disease. Chin. J. Vet. Sci. 20: 324-327.
23. Yang YY, Zhang GP, Guo JQ, Chen FM and Yuan MS. 2000. Preparation and identification of high affinity monoclonal antibodies to IBDV. Chin. J. Immunol. 16: 384-386.
24. Guo JQ, Jin NY, Mao CS, Guo ZR, Yin FG, Yin Z. 1999. High level expression of p24 protein of human immunodeficiency virus type 1 in E. coli. Chin. J. Zoonose 15: 13-16.
25. Yang YY, Zhang GP, Guo JQ and Chen FM. 1999. Effects of the feed cells for culture of the fused cells. Chin. J. Immunol. 15(4): 20-23.
26. Jin NY, Guo JQ, Wang XQ, Tian M, Li TY, Wang HW, Yin FG and Yin Z. 1998. Cloning and expression of p24gag gene of human immunodeficiency virus type 1. High Tech. Commu. 6: 47-51.
27. Mao CS, Jin NY, Tong MH, Guo JQ, Wang XQ and Yin Z. 1998. Effects of long terminal repeat (LTR) of human immunodeficiency virus type 1 on the expression of gag gene in recombinant vaccinia viruses. Virologica Sinica 13: 199-207.
28. Wang HW, Jin NY, Liu Z, Guo JQ, Guo ZR, Mao CS, Li P and Yin Z. 1998. Production and Identification of monoclonal antibody specific for HIV core protein p24. J. Cell Mol. Immuno. 14: 63-64.
29. Mao CS, Jin NY, Tong MH, Chang GQ, Guo JQ, Yang SH, Zou XH and Yin Z. 1998. Expression of HIV-1 Gag protein in Recombinant vaccinia virus and characterization of expressed products. Chin. J. Bio. Agent. 11: 72-75.